Microsatellite Markers in and around Rice Genes: Applications in Variety Identification and DUS Testing

• DOI: 10.2135/cropsci2008.06.0380
• Publication Date: 2009-05-15T01:09:55Z
• Authors: Sandro Bonow; Edila V. R. Von P...; Maria G. C. Vieira; Ben Vosman
• Keywords: UPGMA; Subspecies; Identification; Primer (cosmetics)

In Brazil, rice ( Oryza sativa L.) varieties adapted to different ecological regions are available on the market. However, these exhibit highly similar morphologies, which makes their identification difficult. this study we identified microsatellites in and around genes that useful for of main cultivated Brazil. Thirteen were selected from region upstream MADS‐box 37 derived expressed sequence tags (ESTs). Of 50 selected, 19 polymorphic, a small set five primer pairs was sufficient discriminate among studied. The polymorphic information content (PIC) value microsatellite markers varied between 0.05 0.94. Unweighted pair group method with arithmetic mean (UPGMA) analysis showed according subspecies, indica or japonica These results indicate it is possible identify informative genes, capable discriminating closely related rice. Moreover, our findings suggest can play an important role as source additional supplement morphological descriptors recommended by International Union Protection New Varieties Plants (UPOV).