Identification and Validation of SNP Markers Linked to the Stripe Rust Resistance Gene Yr5 in Wheat

• DOI: 10.2135/cropsci2016.03.0189
• Publication Date: 2016-09-22T20:19:50Z
• Authors: Yukiko Naruoka; Kaori Ando; Peter Bulli; Kebede T. Muleta; Sheri Rynearson; Michael O. Pumphrey
• Keywords: Candidate gene; Common wheat; Brachypodium; Introgression

Stripe rust ( Puccinia striiformis Westend f. sp. tritici ) is a devastating disease for wheat Triticum aestivum L.) production worldwide. Yr5 race‐specific resistance gene, effective to all races that have been identified in the United States. Therefore, it has increasingly used US cultivar development. The goal of this study was identify and validate single‐nucleotide polymorphism (SNP) markers tightly linked with , evaluate their usefulness diverse set materials, dissect region search candidate genes using comparative genomic approach. Competitive allele‐specific polymerase chain reaction (KASP) based on Illumina iSelect 9000 SNP loci IWA6121 IWA4096 were as flanking two spring recombinant inbred line populations developed from crosses between moderately susceptible ‘WA8149’ donors ‘S0900317’ ‘S0900163.’ These closer gene than previously reported markers. Haplotype analysis these KASP Pacific Northwest winter lines worldwide core collection showed combined haplotype nearly diagnostic presence . ‐syntenic regions Brachypodium rice Oryza sativa contained putative encoding nucleotide binding site–leucine‐rich repeat protein. Wheat Traes_2BL_B5FC3BC9E high similarity genes, marker designed mapped closest populations. This provides useful breeding programs resources potential map‐based cloning