Sediment Metagenomics Reveals the Impacts of Poultry Industry Wastewater on Antibiotic Resistance and Nitrogen Cycling Genes in Tidal Creek Ecosystems

• DOI: 10.2139/ssrn.4158230
• Publication Date: 2022-07-10T10:59:17Z
• Authors: Miguel Semedo; Bongkeun Song
• Keywords: Cycling; Nitrogen Cycle

The intensification of the poultry industry may lead to increased spread antibiotic resistance genes (ARGs) in environment. However, impacts wastewater discharge from processing plants on sediment resistome are relatively unexplored. Furthermore, its relationships with important biogeochemical pathways, such as N cycle, virtually unknown. overall objective this study was examine abundance and diversity cycling microbial communities impacted by wastewater. We performed a metagenomic investigation sediments an reference tidal creek. also quantified clinical class 1 integron-integrase gene ( intI1 ) through qPCR secondary marker anthropogenic contamination. Abundance ARGs were substantially higher creek, especially near discharge. Abundances conferring macrolides, tetracyclines, streptogramins creek than From detected metagenomes, nrfA , genetic for dissimilatory nitrate reduction ammonia (DNRA), had strongest positive relationship total ARGs, which indicate potential eutrophication antibiotic-impacted ecosystems due nitrogen retention. This demonstrates that plant can increase result negative ecosystem health.