The Novel Insecticide Broflanilide Dysregulates Transcriptional Networks Associated with Ion Channels and Induces Hyperactivity in Zebrafish (Danio Rerio) Larvae

• DOI: 10.2139/ssrn.4525555
• Publication Date: 2023-07-29T14:22:06Z
• Authors: Sarah Patuel; Cole D. English; Victoria Lopez-Sc...; Isaac Filipe Mor...; Christopher L. S...; Emma Ivantsova; Chris Martyniuk
• Keywords: Danio

Broflanilide is a novel insecticide that classified as non-competitive γ-aminobutyric acid (GABA) receptor antagonist. However, indiscriminate use can have negative effects on non-target species. The objective was to determine the sub-lethal toxicity potential of broflanilide in early staged zebrafish. Embryos/larvae were assessed for multiple molecular and morphological endpoints following exposure different concentrations broflanilide. did not affect hatch rate, frequency deformities, nor it impact survival zebrafish at up 500 μg/L over 7-day period from hatch. There also no effect oxidative consumption rates embryos, induction reactive oxygen species fish exposed 100 As stress prominent mechanism, we turned RNA-seq identify pathways. Gene networks related neurotransmitter release ion channels altered zebrafish, consistent with its mechanism action modulating GABA receptors, which regulate chloride channels. Noteworthy genes circadian clock induced by 1 µg/L exposure. locomotor activity larval 7 days increased (i.e., hyperactivity) based visual motor response test, corroborating expression data indicating neurotoxicity dysfunction. This study improves current understanding biological responses contributes risk assessment strategies this pesticide.