Liquid-Liquid Phase Separation in Subcellular Assemblages and Signaling Pathways: Chromatin Modifications Induced Gene Regulation for Cellular Physiology and Functions Including Carcinogenesis

• DOI: 10.2139/ssrn.4603432
• Publication Date: 2023-10-19T21:22:06Z
• Authors: Subhajit Chakraborty; Jagdish Mishra; Ankan Roy; Niharika Niharika; Soumen Manna; Tirthankar Baral; Piyasa Nandi; Subhajit Patra; SAMIR KUMAR PATRA
• Keywords: Organelles; Phase Separation; Carcinogenesis; Neoplasms; Humans; Animals; Chromatin Assembly and Disassembly; Chromatin; Signal Transduction; Epigenesis, Genetic

Liquid-liquid phase separation (LLPS) describes many biochemical processes, including hydrogel formation, in the integrity of macromolecular assemblages and existence membraneless organelles, ribosome, nucleolus, nuclear speckles, paraspeckles, promyelocytic leukemia (PML) bodies, Cajal bodies (all exert crucial roles cellular physiology), evidences are emerging day by day. Also, is well documented generation plasma membrane subdomains interplay between membranous organelles. Intrinsically disordered regions (IDRs) biopolymers/proteins most critical sticking that aggravates formation such condensates. Remarkably, separated condensates also involved epigenetic regulation gene expression, chromatin remodeling, heterochromatinization. Epigenetic marks on DNA histones cooperate with RNA-binding proteins through their IDRs to trigger LLPS for facilitating transcription. How coalesces mutant oncoproteins, orchestrate tumor suppressor genes facilitated cancer-associated signaling pathways unravelling. That autophagosome DYRK3-mediated cancer stem cell modification depend deciphered part. In view this, linchpin insight into subcellular organelle assembly, activation biological reactions catalyzed enzymes, downstream physiological functions, how all these events precisely inducing function, modulation expression this scenario, it goes awry progression summarized presented article.