Specific Fluorescence Analysis of Veterinary Drugs in Animal Products

• DOI: 10.2139/ssrn.4673919
• Publication Date: 2023-12-23T02:48:15Z
• Authors: Milada Vodova; Kristyna Pavelicova; Kristyna Veverkova; Lukas Nejdl; Tomas Rypar; Jaroslava Bezdekova; Petr Maly; Veronika Vodova; Vojtech Adam; Marketa Vaculovicova
• Keywords: Veterinary Drugs; Veterinary Drugs; Animal health

Balancing the benefits of veterinary drugs, such as antibiotics, in food production with risks antibiotic resistance is an ongoing topic debate. Here, we utilized UV-induced fingerprint Spectroscopy (UV-IFS) to conduct a photochemical analysis 22 UV-IFS allows for monitoring changes spectral properties before and after exposure ultraviolet radiation (λ = 254 nm). This initiates reactions, resulting distinctive alterations enabling sample qualitative quantitative identification. Through this analysis, optimized method analyzing samples (Ciplox Betamox L.A.) containing ciprofloxacin (CIP) amoxicillin (AMX), achieving limit detection 18 ng/mL CIP 4 μg/mL AMX. Furthermore, successfully detected at 61 concentration milk sample, employing molecularly imprinted polymers (MIP). MIP-based approach allowed specific isolation from matrix, which was subsequently evaluated using UV-IFS.