Transcriptomic and Metabolomic Profilings Reveal the Accumulation of Related Metabolites and Gene Regulatory Networks of Quinoa Leaves to Spodoptera Exigua Feeding During Flowering Periods

• DOI: 10.2139/ssrn.5087488
• Publication Date: 2025-01-08T14:21:16Z
• Authors: Junna Liu; Jian Liu; Ping Zhang; Qianchao Wang; Li Li; Hanxue Li; Peng Qin; Shunhe Cheng
• Keywords: Exigua

Background: The transcriptional response of plants to insect attacks involves dynamic and complex signaling synthetic reprogramming, which change over time. Spodoptera exigua, an important pest, poses a threat quinoa, affecting its quality. Results: This study aimed elucidate the defense quinoa transcriptome S.exigua by subjecting leaves nibbling for 5, 15, 30, 60 min. metabolite profiles these samples were compared those control group without activity. After 5 min, both gene expression changed significantly. When fed on 15 stronger faster occurred in defense-related LOC110682359 LOC110701735. Conclusions: As quercetin content gradually increased with longer feeding durations. In contrast, jasmonic acid levels lowest at min but subsequently increased. KEGG pathway analysis revealed that after nibbling, DEGs implicated plant hormone signal transduction, alpha-linolenic metabolism, phenylpropanoid biosynthesis, related resistance S.exigua. WGCNA showed LOC110701735 closely acid, two homologous genes AtMYB44 significantly induced when Subcellular localization indicates proteins located nucleus belong nuclear proteins. Overexpression highly expressed transgenic Arabidopsis, higher than wild-type Arabidopsis. results successfully inserted into Arabidopsis genome normally, regulating JA mediated feeding. summary, provide comprehensive view metabolic responses