Global Analysis of RNA Oxidation in Saccharomyces Cerevisiae

• DOI: 10.2144/000113801
• Publication Date: 2012-11-28T19:26:51Z
• Authors: Anastasia McKinlay; Wayne Gerard; Stanley Fields
• Keywords: 0303 health sciences; RNA oxidation; Saccharomyces cerevisiae Proteins; 8-OHG modification; QH301-705.5; free radicals; RNA, Fungal; Saccharomyces cerevisiae; yeast; RNA modification; High-Throughput Screening Assays; 03 medical and health sciences; Biology (General); Oxidation-Reduction

Oxidative RNA damage has been linked to loss of function and the development many human age-related diseases. Consequently, a need exists for methods identify quantify extent oxidation on genome-wide basis. We developed such method by combining affinity selection mRNA containing 8-hydroxyguanine with high throughput DNA sequencing. demonstrate that this assay is suitable detecting differences in between transcripts. applied yeast Saccharomyces cerevisiae grown under physiological conditions response hydrogen peroxide, detected significantly oxidized