Single Step BP/LR Combined Gateway Reactions
Recombination, Genetic
0301 basic medicine
one-pot reaction
QH301-705.5
Genetic Vectors
cloning
DNA
Bacteriophage lambda
recombination
03 medical and health sciences
clonase
Biology (General)
Cloning, Molecular
Plasmids
DOI:
10.2144/000114101
Publication Date:
2016-07-06T04:27:36Z
AUTHORS (4)
ABSTRACT
The Gateway recombination system is characterized by its ability to transfer DNA sequences back and forth between an intermediate clone (the entry clone) and a variety of destination vectors. However, a number of applications do not need to exploit the advantages offered by the entry clone. Here we report reaction conditions for cloning DNA fragments into destination vectors in a single step reaction, thus reducing the cost and overall time needed to obtain an expression clone from three days to one.
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