Enhancing Colorimetric Loop-mediated Isothermal Amplification Speed and Sensitivity with Guanidine Chloride

• DOI: 10.2144/btn-2020-0078
• Publication Date: 2020-07-08T07:42:43Z
• Authors: Yinhua Zhang; Guoping Ren; Jackson Buss; Andrew J Barry; Gregory C Patton; Nathan A Tanner
• Keywords: Guanidine; Primer (cosmetics); Hydrochloride; Colorimetry

Loop-mediated isothermal amplification (LAMP) is a versatile technique for detection of target DNA and RNA, enabling rapid molecular diagnostic assays with minimal equipment. The global SARS-CoV-2 pandemic has presented an urgent need new better methods, colorimetric LAMP utilized in numerous studies detection. However, the sensitivity early reports been below that standard RT-qPCR tests, we sought to improve performance. Here report use guanidine hydrochloride combined primer sets increase speed LAMP, bringing this simple method up standards sophisticated techniques accurate, high-throughput diagnostics.