Crystal Violet Staining is a Reliable Alternative to Bicinchoninic Acid Assay-Based Normalization

• DOI: 10.2144/btn-2022-0064
• Publication Date: 2022-08-24T08:18:10Z
• Authors: Julie Nilles; Johanna Weiss; Dirk Theile
• Keywords: Crystal violet; Bicinchoninic acid assay; Normalization

Experimental data with cells often require normalization. The frequently used bicinchoninic acid (BCA) assay, in fact, indicates protein content but is influenced by incubation time, pH etc. A simple, rapid and reliable alternative desirable. Crystal violet stains nucleic acids proteins was to reflect the cell number 96-well plates. Calibration curves comparison BCA confirmed excellent goodness of fit (R2: 0.98), conformity (nonsignificant difference crystal violet) reliability this staining methodology. can be normalize experimental adherent present culture