A Novel Nano-Hydroxyapatite Agarose-Based Hydrogel for Biomimetic Remineralization of Demineralized Human Enamel: An in-vitro Study

• DOI: 10.2147/ccide.s478045
• Publication Date: 2024-11-02T09:35:12Z
• Authors: Sara El Moshy; Israa Radwan; Manal Matoug-Elwe...; Ahmed Abdou; Marwa Abbass
• Keywords: Agarose

Purpose: This study aims to investigate the biomimetic effect of agarose hydrogel loaded with enamel matrix derivative (EMD-agarose) alone or in combination nano-hydroxyapatite (n-HA-EMD-agarose) on remineralization human demineralized enamel. Methods: Extracted mandibular third molars were sectioned into 54 buccal and lingual halves. Acid-resistant nail varnish was applied each half, except for two windows. Enamel surface microhardness, energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM) analyses conducted evaluate surfaces at baseline, following demineralization 37% phosphoric acid, after application two, four, six days. Remineralization performed using a phosphate solution 37°C. Results: At day 6 remineralization, statistically significant higher mean microhardness recorded n-HA-EMD-agarose (260.87 ± 3.52) as compared EMD-agarose (244.63 2.76) (p = 0.027). Similarly, showed calcium (46.31 2.78), phosphorous (24.92 0.826), fluoride (0.909 0.053) weight percentage (19.64 1.092), (0.7033 0.0624) < 0.05). Further, SEM analysis revealed substantial deposition n-HA hydrogel, whereas exhibited relatively smooth less visible rods due mineral deposition. Conclusion: The combined demonstrated improved remineralized enhanced content deposition, indicating its potential approach dental repair. Keywords: biomimetic, derivatives, nano-hydroxy apatite,