<p>SNHG1 contributes to proliferation and invasion by regulating miR-382 in breast cancer</p>

• DOI: 10.2147/cmar.s198624
• Publication Date: 2019-06-17T22:54:31Z
• Authors: Shipeng Zheng; Mengquan Li; Keke Miao; Han Xu
• Keywords: 0303 health sciences; 03 medical and health sciences; breast cancer; miR-382; Cancer Management and Research; therapeutic target; Neoplasms. Tumors. Oncology. Including cancer and carcinogens; SNHG1; RC254-282; Original Research; 3. Good health

Introduction: Long non-coding RNAs (lncRNAs) are key regulators in multiple cancers. lncRNA, SNHG1, was shown to be associated with tumorigenesis. However, little is known about the role SNHG1 plays breast cancer. The aim of study and underlying mechanism regulation Methods: Quantitative real-time PCR used measure levels miR-382 ZEB1 cancer tissue or cells. proliferation, colony formation, migration invasion cells, under knockdown achieved by transfection SNHG1-specific siRNAs, were assessed Cell Counting Kit-8, forming, scratch wound transwell assays. Bioinformatical analysis luciferase assay explore interaction between its potential miRNA target. Western blot evaluate expression epithelial-to-mesenchymal transition (EMT) markers. MDA-MB-231 cells without initiate tumor xenografts vivo. Tumor growth miR-382-5p EMT markers evaluated. Results: upregulation observed tissues Knockdown attenuated invasion. A miRNA, miR-382-5p, identified as target SNHG1. reciprocal negative found miR-382-5p. both vitro reversed tumor-promoting In vivo, decreased growth. Conclusion: promotes through Our results report a novel that govern progression cancer, providing new therapeutic