<p>Differential gene expression identifies KRT7 and MUC1 as potential metastasis-specific targets in sarcoma</p>

• DOI: 10.2147/cmar.s218676
• Publication Date: 2019-09-08T22:44:36Z
• Authors: Long Jiang; Bhairavi Tolani; Che-Chung Yeh; Yanying Fan; Joseph A Reza; Andrew Horvai; Endi Xia; Johannes R. Kratz; David M. Jablons; Michael J. Mann
• Keywords: MUC1

Despite numerous discoveries regarding the molecular genesis and progression of primary cancers, biology metastasis remains poorly understood. Compared to very large numbers circulating tumor cells that are now known accompany nearly all a relatively limited number lesions actually develop in most patients with metastases. We hypothesized phenotypic changes driven by differential gene expression finite subpopulation render those capable sought identify key pathways through analysis metastatic from same patients.We compared whole-genome 4 matched samples sarcoma, then evaluated candidate genes via quantitative PCR 30 additional sets, tissue immunostaining, siRNA loss-of-function sarcoma cell migration assay, clinical correlation overall disease-free survival after metastasectomy.Comparison microarray signals identified adhesion genes, including upregulation KRT7 MUC1 metastases; was confirmed 22 (73%) 20 (67%) sets metastatic/primary tumors, respectively. Silencing targeted siRNAs suppressed vitro, significant (two-sided) observed between both metastases patient survival.KRT7 may play role enabling metastasis, they therefore be important prognostic biomarkers as well potential targets for therapeutic prevention metastasis.