<p>Paeonol Inhibits Pancreatic Cancer Cell Migration and Invasion Through the Inhibition of TGF-β1/Smad Signaling and Epithelial-Mesenchymal-Transition</p>

• DOI: 10.2147/cmar.s224416
• Publication Date: 2020-01-29T00:45:30Z
• Authors: Chien-Shan Cheng; Jing-Xian Chen; Jian Tang; Ya-Wen Geng; Lan Zheng; Ling-Ling Lv; Lian-Yu Chen; Zhen Chen
• Keywords: Paeonol; MTT assay

Paeonol, a natural product derived from the root of Cynanchum paniculatum (Bunge) K. Schum and Paeonia suffruticosa Andr. (Ranunculaceae) has attracted extensive attention for its anti-cancer proliferation effect in recent years. The present study examined role paeonol suppressing migration invasion pancreatic cancer cells by inhibiting TGF-β1/Smad signaling.Cell viability was evaluated MTT colonial formation assay. Migration capabilities were cell scratch-wound healing assay Boyden chamber Western Blot qRT-PCR used to measure protein RNA levels vimentin, E-cadherin, N-cadherin, signaling.At non-cytotoxic dose, 100 μΜ 150 showed significant anti-migration anti-invasion effects on Panc-1 Capan-1 (p<0.01). Paeonol inhibited epithelial-mesenchymal-transition upregulating down regulating N-cadherin vimentin expressions. signaling pathway downregulating TGF-β1, p-Smad2/Smad2 p-Smad3/Smad3 Further, TGF-β1 attenuated capacities cells.These findings revealed that could suppress inhibit might be promising novel anti-pancreatic drug.