<p>Long Non-Coding RNA LINC00511 Mediates the Effects of ESR1 on Proliferation and Invasion of Ovarian Cancer Through miR-424-5p and miR-370-5p</p>

• DOI: 10.2147/cmar.s232140
• Publication Date: 2019-12-26T16:52:15Z
• Authors: Kang Wang; Genhai Zhu; Shan Bao; Shiling Chen
• Keywords: Estrogen receptor alpha; Fulvestrant

Estrogen receptor 1 (ESR1) plays an important role in the pathological events of ovarian cancer (OV), but underlying mechanism is not completely understood. Using bioinformatics analysis, we found that ESR1 involved regulation some lncRNAs are highly expressed cancer. The might mediate roles OV occurrence and progression.This study measured expression cell lines using qRT-PCR. Some were silenced or overexpressed to determine their effects on growth invasion CAOV3 cells with stimulation 17 beta-estradiol not.ESR1-expressing (CAOV3 cells) shows higher LINC00511 RP11-166P13.3 than ESR1-losing (UWB1.289 cells). Depletion two enhanced viability decreased apoptosis rate. In these respects, more remarkable those RP11-166P13.3. Treatment stimulate increased expression, while inhibitor Fulvestrant expression. FISH assay confirmed present cytoplasm nucleus. Bioinformatics analysis revealed interaction miR-424-5p miR-370-5p, which was further identified by RNA-pull down assay. As indicated RIP assay, silencing between Ago protein miRNAs.Our showed ESR1-induced upregulation promoted proliferation probably through sponging miR-370-5p.