<p>Evaluation of Concordance Between Deficient Mismatch Repair and Microsatellite Instability Testing and Their Association with Clinicopathological Features in Colorectal Cancer</p>

• DOI: 10.2147/cmar.s248069
• Publication Date: 2020-04-23T21:59:28Z
• Authors: Huili Bai; Rong Wang; Wei Cheng; Yifan Shen; Haijun Li; Wei Xia; Zhenglin Ding; Yuhong Zhang
• Keywords: Microsatellite Instability; Concordance; PMS2; Lynch Syndrome

Microsatellite instability (MSI) is one of the most important molecular characteristics colorectal cancer (CRC), which mainly results from defective DNA mismatch repair (MMR). This study was performed to investigate concordance between deficient MMR and MSI testing, evaluate association these two with clinicopathological in Chinese CRC patients.A total 738 patients were included. Tumor tissues paired peripheral blood specimens obtained. Screening for investigated using immunohistochemical (IHC) technique, multiple polymerase chain reaction-capillary electrophoresis (PCR-CE) method detect status. All data, immunohistochemistry microsatellite analyses then statistically analyzed.Of (17.75%) patients, 131 expressed as (dMMR) status, postmeiotic segregation increased 2 (PMS2) deficiency frequent among four proteins. MSI-high (MSI-H) status occurred 74 (10.03%) 55 whom showed at all six mononucleotides repeat markers. dMMR significantly associated MSI-H moderate observed IHC PCR-CE evaluating MMR/MSI through Kappa test. Statistically, younger age, right-sided colon poor differentiation. colon, differentiation, mucinous type tumor, node, metastasis (TNM) stage II.A testing indicates that both methods should be routinely tested provide reliable data clinical treatment decisions.