<p>miR-100-5p Inhibits Malignant Behavior of Chordoma Cells by Targeting IGF1R</p>

• DOI: 10.2147/cmar.s252185
• Publication Date: 2020-06-01T22:59:18Z
• Authors: Hongliang Zhang; Kang Yang; Tingting Ren; Yi Huang; Xin Liang; Yiyang Yu; Wei Wang; Jianfang Niu; Jingbing Lou; Xiaodong Tang; Wei Guo
• Keywords: Chordoma

Our research aimed to illuminate the role of miR-100-5p in chordoma and potential mechanism.We used microRNA array analysis explore differentially expressed miRNAs tissue then verified by qRT-PCR. Cell proliferation transwell assay were evaluate function miR-100-5p. apoptosis was analyzed flow cytometry, using biological software, we predicted that insulin-like growth factor 1 receptor (IGF1R) could be target gene miR-100-5p, which validated dual luciferase assays Western blot.miR-100-5p downregulated tissues. Overexpression suppress both vitro vivo, inhibit migration invasion cells partially suppressing epithelial-mesenchymal transition (EMT). Furthermore, IGF1R as most tissues.In conclusion, our results showed lowly inhibited tumor malignant progression targeting IGF1R.