Identification of Ferroptosis-Related Hub Genes and Their Association with Immune Infiltration in Chronic Obstructive Pulmonary Disease by Bioinformatics Analysis

• DOI: 10.2147/copd.s348569
• Publication Date: 2022-05-24T09:21:21Z
• Authors: Yi-Can Yang; Meng-Yu Zhang; Jian-Yu Liu; Yuan-Yuan Jiang; Xiu-Li Ji; Yi-Qing Qu
• Keywords: KEGG; Fold change; Pathogenesis; Infiltration (HVAC)

Ferroptosis and immune infiltration are involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). We aim to identify ferroptosis-related hub genes analyze their association with COPD through bioinformatics methods.The mRNA microarray data GSE38974 were downloaded from Gene Expression Omnibus obtain differentially expressed (DEGs). The DEGs intersected (FRGs) FerrDb FRGs. GO KEGG enrichment protein-protein interaction (PPI) analyses FRGs conducted R software STRING database. key module screened by Cytoscape software. MiRNAs, transcription factors signal molecules predicted miRNet NetworkAnalyst. correlation Comparative Toxicomics Database (CTD) receiver operating characteristic (ROC) curves analyzed. Immune was evaluated CIBERSORT algorithm. Spearman between infiltrated cells.Fifteen identified, which enriched some terms involving airway inflammatory responses structural remodeling. Five selected including HIF1A, IL6, PTGS2, CDKN1A ATM. Inference scores CTD indicated COPD. Two miRNAs, five one molecule predicted. combination could be a fine diagnostic indicator (AUC: 0.981, CI: 0.940-1.000). evaluation showed that monocytes M0 macrophages upregulated lung tissues, while CD8 T cells, activated NK M2 macrophages, resting dendritic cells mast downregulated. significantly associated cells.We identified (HIF1A, ATM) COPD, found they may influence regulating ferroptosis thus affecting infiltrating cells.