Fabrication of novel vesicles of triptolide for antirheumatoid activity with reduced toxicity in vitro and in vivo

• DOI: 10.2147/ijn.s104593
• Publication Date: 2016-06-09T01:11:09Z
• Authors: Qianqian Liang; Li Zhang; Tengteng Wang; Qiang Li; Jing Huang; Hao Xu; Jinlong Li; Yongjun Wang
• Keywords: Blood urea nitrogen; Triptolide

Abstract: Triptolide (TP) displays a strong immunosuppression function in immune-mediated diseases, especially the treatment of rheumatoid arthritis. However, addition to its medical and health-related functions, TP also exhibits diverse pharmacological side effects, for instance, liver kidney toxicity myelosuppression. In order reduce nano drug carrier system (γ-PGA-l-PAE-TP [PPT]), which was loaded by poly-γ-glutamic acid-grafted l-phenylalanine ethylester copolymer, developed. PPT characterized photon scattering correlation spectroscopy transmission electron microscopy, demonstrated that average diameter is 98±15 nm, polydispersity index 0.18, zeta potential –35 mV, encapsulation efficiency 48.6% with controlled release manner. The methylthiazolyldiphenyl-tetrazolium bromide assay flow cytometry revealed could decrease apoptosis induced free on RAW264.7 cells, respectively. detection reactive oxygen species showed cellular TP. Compared TP-treated group, improved survival rate mice ( P <0.01) had no effects or toxic thymus >0.05) spleen >0.05). blood biochemical indexes did not cause much damage (blood urea nitrogen creatinine), (serum alanine aminotransferase aspartate aminotransferase), cells Meanwhile, hematoxylin eosin staining terminal-deoxynucleotidyl transferase dUTP nick-end labeling indicated reduced liver, kidney, spleen. Our results vitro vivo it promising fundamental delivery arthritis treatment. Keywords: triptolide, toxicity, acid, Corrigendum this paper has been published