Surface plasmon-enhanced fluorescence on Au nanohole array for prostate-specific antigen detection

• DOI: 10.2147/ijn.s128172
• Publication Date: 2017-03-28T00:50:17Z
• Authors: Qingwen Zhang; Lin Wu; Ten It Wong; Jinling Zhang; Xiaohu Liu; Xiaodong Zhou; Ping Bai; Bo Liedberg; Yi Wang
• Keywords: Immunoassay; Medicine (General); localized surface plasmon; Gold nanohole array; fluorescence enhancement; Metal Nanoparticles; Localized surface plasmon; gold nanohole array; 02 engineering and technology; Prostate-Specific Antigen; Surface Plasmon Resonance; R5-920; Spectrometry, Fluorescence; Electricity; International Journal of Nanomedicine; propagating surface plasmon; Limit of Detection; prostate-specific antigen; Humans; Computer Simulation; Gold; 0210 nano-technology; Original Research

Localized surface plasmon (LSP) has been widely applied for the enhancement of fluorescence emission biosensing owing to its potential strong field enhancement. However, due small penetration depth, LSP offers limited over a whole sensor chip and, therefore, insufficient sensitivity detection biomolecules, especially large molecules. We demonstrate simultaneous excitation and propagating (PSP) on an Au nanohole array under Kretschmann configuration prostate-specific antigen with sandwich immunoassay. The proposed method combines advantages high by area probed PSP field. simulated results indicated that maximum electric intensity up 1,600 times can be achieved modes. assay PSA carried out gold substrate showed limit 140 fM supporting coexcitation was approximately sevenfold lower than when only resonantly excited same substrate. this study through modes pave way implementation as highly sensitive bioassay.