Detection of influenza viruses by coupling multiplex reverse-transcription loop-mediated isothermal amplification with cascade invasive reaction using nanoparticles as a sensor

• DOI: 10.2147/ijn.s132670
• Publication Date: 2017-04-03T19:31:19Z
• Authors: Yiyue Ge; Qiang Zhou; Kangchen Zhao; Ying Chi; Bin Liu; Xiaoyan Min; Zhiyang Shi; Bingjie Zou; Lunbiao Cui
• Keywords: Multiplex

Abstract: Influenza virus infections represent a worldwide public health and economic problem due to the significant morbidity mortality caused by seasonal epidemics pandemics. Sensitive convenient methodologies for detection of influenza viruses are essential further disease control. Loop-mediated isothermal amplification (LAMP) is most commonly used method nucleic acid amplification. However, with regard multiplex LAMP, differentiating ladder-like LAMP products derived from multiple targets still challenging today. The requirement specialized instruments has hindered on-site application LAMP. We have developed an integrated assay coupling reverse transcription cascade invasive reaction using nanoparticles (mRT-LAMP-CIRN) as sensor three subtypes viruses: A/H1N1pdm09, A/H3 B. analytic sensitivities mRT-LAMP-CIRN were 10 1 copies RNA both A/H1N1pdm09 A/H3, 2 This demonstrated highly specific target could differentiate them other genetically or clinically related viruses. Clinical specimen analysis showed had overall sensitivity specificity 98.3% 100%, respectively. In summary, sensitive specific, can be cost-saving instrument-free viruses, especially use. Keywords: multiplex, gold nanoparticles, detection,