Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo

• DOI: 10.2147/ijn.s134897
• Publication Date: 2017-06-13T21:43:28Z
• Authors: Zhen Yan; Wenjun Wang; Yongjun Wu; Wei Wang; Bing Li; Ning Liang; Weidong Wu
• Keywords: Proinflammatory cytokine

Engineered zinc oxide nanoparticles (ZnO-NPs) are currently being produced in high tonnage. Exposure to ZnO-NPs presents potential risks cardiovascular system. Thus far, the toxicological effects of on system have not been well characterized. In this study, human coronary artery endothelial cells (HCAECs) were exposed directly or indirectly using a transwell coculture with alveolar epithelial cell line A549 mimic lung/circulation interaction. It was shown that levels proinflammatory mediators (interleukin-8 [IL-8] and tumor necrosis factor-α [TNF-α]) biomarkers atherosclerogenesis (heme oxygenase-1 [HO-1] platelet adhesion molecules-1 [PECAM-1]) supernatants culture media significantly increased. Pretreatment apical side phagocytosis inhibitor cytochalasin B (CB) blocked ZnO-NP-induced HO-1 PECAM-1 expression HCAEC, indicating endocytosis by involved cells. Moreover, Wistar rats intratracheally instilled ZnO-NP suspension fat diet (positive control). treatment induced lung systemic inflammation, dyslipidemia, increased serum PECAM-1, aortic pathological damage. Taken together, exposure could induce atherosclerotic alterations, which might involve inflammation lung.