Guided bone regeneration with asymmetric collagen-chitosan membranes containing aspirin-loaded chitosan nanoparticles

• DOI: 10.2147/ijn.s148179
• Publication Date: 2017-12-15T00:58:04Z
• Authors: Jiayu Zhang; Shiqing Ma; Zihao Liu; Hongjuan Geng; Xin Lu; Xi Zhang; Hongjie Li; Chenyuan Gao; Xu Zhang; Ping Gao
• Keywords: Medicine (General); Bone Regeneration; aspirin; 12. Responsible consumption; Rats, Sprague-Dawley; 03 medical and health sciences; R5-920; Drug Delivery Systems; Microscopy, Electron, Transmission; International Journal of Nanomedicine; Osteogenesis; Materials Testing; Animals; membrane; drug release; Original Research; Chitosan; 0303 health sciences; Aspirin; nanoparticle; Membranes, Artificial; Mesenchymal Stem Cells; X-Ray Microtomography; 3. Good health; Drug Liberation; guided bone regeneration; Microscopy, Electron, Scanning; Nanoparticles; Collagen

Membranes allowing the sustained release of drugs that can achieve cell adhesion are very promising for guided bone regeneration. Previous studies have suggested aspirin has potential to promote The purpose this study was prepare a local drug delivery system with aspirin-loaded chitosan nanoparticles (ACS) contained in an asymmetric collagen-chitosan membrane (CCM).In study, ACS were fabricated using different concentrations (5 mg, 25 50 and 75 mg). behavior studied. Transmission electron microscopy (TEM) scanning (SEM) used examine micromorphology chitosan-collagen membranes (ACS-CCM). In vitro mesenchymal stem cells (BMSCs) cultured critical-sized cranial defects on Sprague-Dawley rats made evaluate effect ACS-CCM regeneration.Drug results showed could successfully sustain drug. TEM morphology nanoparticles. SEM images indicated comprised loose collagen layer dense layer. proliferation BMSCs, degree differentiated BMSCs seeded CCMs containing mg higher than other membranes. Micro-computed tomography resulted enhanced regeneration compared control group.This shows would allow further osteogenic potential. This is therapeutic approach guiding