Biosynthesis of Au, Ag and Au&ndash;Ag bimetallic nanoparticles using protein extracts of <em>Deinococcus radiodurans</em> and evaluation of their cytotoxicity

• DOI: 10.2147/ijn.s149079
• Publication Date: 2018-03-08T22:09:10Z
• Authors: Jiulong Li; Bing Tian; Tao Li; Shang Dai; Yulan Weng; Jianjiang Lu; Xiaolin Xu; Ye Jin; Renjiang Pang; Yuejin Hua
• Keywords: Zeta potential; Bimetallic strip

Background: Biosynthesis of noble metallic nanoparticles (NPs) has attracted significant interest due to their environmental friendly and biocompatible properties. Methods: In this study, we investigated syntheses Au, Ag Au–Ag bimetallic NPs using protein extracts Deinococcus radiodurans , which demonstrated powerful metal-reducing ability. The obtained were characterized analyzed by various spectroscopy techniques. Results: D. extract-mediated silver (Drp-AgNPs) preferably monodispersed stably distributed compared gold (Drp-AuNPs). Drp-AgNPs Drp-AuNPs exhibited spherical morphology with average sizes 37.13±5.97 nm 51.72±7.38 zeta potential values -18.31±1.39 mV -15.17±1.24 at pH 7, respectively. release efficiencies measured 24 h 3.99% 18.20%, During the synthesis process, Au(III) was reduced Au(I) further Au(0) Ag(I) Ag(0) interactions hydroxyl, amine, carboxyl, phospho or sulfhydryl groups proteins subsequently stabilized these groups. Some characteristics different from those Drp-AgNPs, could be attributed interaction binding proteins. formed into via galvanic replacement reaction. showed low cytotoxicity against MCF-10A cells lower level intracellular reactive oxygen species (ROS) generation than that Drp-AgNPs. Conclusions: These results are crucial understand biosynthetic mechanism properties bacteria. Au applicable in biosensing, bioimaging biomedicine. Keywords: extreme bacterium, green synthesis, nanoparticles,