Nanostructured titanium regulates osseointegration via influencing macrophage polarization in the osteogenic environment

• DOI: 10.2147/ijn.s163956
• Publication Date: 2018-07-10T00:42:05Z
• Authors: Jinjin Wang; Fanhui Meng; Wen Song; Jingyi Jin; Qianli Ma; Dongdong Fei; Liang Fang; Lihua Chen; Qintao Wang; Yumei Zhang
• Keywords: Macrophage polarization; Biocompatibility

Fabricating nanostructured surface topography represents the mainstream approach to induce osteogenesis for next-generation bone implant. In past, implant was designed minimize host repulsive reactions in order acquire biocompatibility. However, increasing reports indicate that absence of an appropriate immune response cannot adequate osseointegration after implantation vivo.We prepared different topographies on titanium (Ti) specimens by grinding, etching and anodizing, they were marked as polished specimen (P), with nanotubes (NTs) small diameters (NT-30) NTs large (NT-100). We evaluated ability osteogenic differentiation mice marrow mesenchymal stem cells (BMSCs) vitro vivo. Furthermore, we investigated effect polarization secretion macrophages, macrophage topography-induced BMSCs. Finally, verified vivo using Cre*RBP-Jfl/fl which classically activated restrained.The BMSCs induced NT-100>NT-30>P, while NT-30>NT-100>P. addition, NT-30 could more macrophages M2 polarization, P NT-100 M1 polarization. When co-culture topographies, NT-30>NT-100≥P. The much better than wild type mice.It is suggested intrinsic immunomodulatory effects nanomaterials are not only crucial evaluate biocompatibility but also required determine final osseointegration. To clarify may be beneficial designation optimization