Macroporous nanofibrous vascular scaffold with improved biodegradability and smooth muscle cells infiltration prepared by dual phase separation technique

• DOI: 10.2147/ijn.s183463
• Publication Date: 2018-10-31T21:36:59Z
• Authors: Weizhong Wang; Wei Nie; Dinghua Liu; Haibo Du; Xiaojun Zhou; Liang Chen; Hongsheng Wang; Xiumei Mo; Lei Li; Chuanglong He
• Keywords: PLGA; Interconnectivity; Electrospinning; Miscibility

Macroporous nanofibrous vascular scaffold with improved biodegradability and smooth muscle cells infiltration prepared by dual phase separation technique Weizhong Wang,1 Wei Nie,1 Dinghua Liu,1 Haibo Du,1 Xiaojun Zhou,1 Liang Chen,1 Hongsheng Xiumei Mo,1 Lei Li,2 Chuanglong He1 1Key Laboratory of Science & Technology Eco-Textiles, Ministry Education, College Chemistry, Chemical Engineering Biotechnology, Donghua University, Shanghai 201620, China; 2Department Gastroenterology, Ninth People’s Hospital Affiliated to Jiao Tong University School Medicine, 200011, China Introduction: The fast degradation graft the (SMCs) into are considered be critical for regeneration functional neo-vessels. In our previous study, a novel was developed one-pot prepare macroporous poly(l-lactic acid) (PLLA)/poly(ε-caprolactone) (PCL) separating immiscible polymer blend. However, slow PLLA/PCL limited cell infiltration. Herein, we hypothesized that poly(lactic-co-glycolic (PLGA) would miscible PLLA but PCL. Then, PLGA can introduced blend fabricate using technique. Materials methods: miscibility PCL evaluated. PLLA/PLGA/PCL scaffolds were characterized in terms morphology, vitro degradation, mechanical properties, cells’ viability human SMCs (HVSMCs). Finally, platelet-derived growth factor-BB (PDGF-BB) immobilized on its effect bioactivity HVSMCs studied. Results: is as hypothesized. addition enlarged pore size composite scaffold. Notably, ratio 30:40:30 possessed interconnectivity enough properties. Moreover, could grow infiltrate this scaffold, surface modification PDGF-BB enhanced migration proliferation. Conclusion: This study provides strategy expand utilizing ternary even multinary physicochemical promising tunica media tissue engineering. Keywords: blend, porous, nanofibrous,