Injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration

• DOI: 10.2147/ijn.s185715
• Publication Date: 2018-10-31T18:50:03Z
• Authors: Donghyun Lee; Dong Nyoung Heo; Ha Ram Nah; Sang Jin Lee; Wan-Kyu Ko; Jae Seo Lee; Ho-Jin Moon; Jae Beum Bang; Yu-Shik Hwang; Rui L. Reis; Il Keun Kwon
• Keywords: 0301 basic medicine; Bone Regeneration; Cell Survival; Stem Cells; Metal Nanoparticles; Cell Differentiation; Hydrogels; Alkaline Phosphatase; Bone and Bones; Acetylcysteine; Injections; 3. Good health; 03 medical and health sciences; Adipose Tissue; International Journal of Nanomedicine; Osteogenesis; Gelatin; Humans; Gold; Original Research

For effective bone regeneration, it is necessary to implant a biocompatible scaffold that capable of inducing cell growth and continuous osteogenic stimulation at the defected site. Here, we suggest an injectable hydrogel system using enzymatic cross-linkable gelatin (Gel) functionalized gold nanoparticles (GNPs).In this work, tyramine (Ty) was synthesized on backbone (Gel-Ty) enable phenol crosslinking reaction with horseradish peroxidase (HRP). N-acetyl cysteine (NAC) attached GNPs surface (G-NAC) for promoting osteodifferentiation.The Gel-Ty hydrogels containing G-NAC (Gel-Ty/G-NAC) had suitable mechanical strength biocompatibility embed support human adipose derived stem cells (hASCs) during proliferation test three days. In addition, promoted osteodifferentiation both when included in used directly hASCs. The effects were demonstrated by alkaline phosphatase (ALP) activity test.These findings indicate tissue regeneration stimulate regeneration. Based our results, Gel-Ty/G-NAC can serve as biodegradable graft material defect treatment good template engineering applications such drug delivery, various uses.