<p>Stability and osteogenic potential evaluation of micro-patterned titania mesoporous-nanotube structures</p>

• DOI: 10.2147/ijn.s199610
• Publication Date: 2019-06-04T20:33:15Z
• Authors: Xi Ding; Yuzhen Wang; Lihua Xu; Hualin Zhang; Zhennan Deng; Lina Cai; Zuosu Wu; Litao Yao; Xinghai Wu; Jinsong Liu; Xinkun Shen
• Keywords: Anodizing

Background: Although titanium dioxide nanotubes (TNTs) had great potential to promote osteogenesis, their weak bonding strength with substrates greatly limited clinical application. Purpose: The objective of this study was maintain porosity and improve the stability TNT coatings by preparing some micro-patterned mesoporous/nanotube (MP/TNT) structures via a photolithography-assisted anodization technology. Methods: adhesion different studied ultrasonic cleaning machine scratch tester. early adhesion, spreading, proliferation differentiation MC3T3-E1 cells on were investigated in vitro fluorescent staining, CCK8, alkaline phosphatase activity, mineralization polymerase chain reaction assays, respectively. Results: Results assays showed that TNTs (especially 125 nm) significantly improved after being patterned MP structures. In cell further demonstrated insertion structure into nm coating, which denoted as MP125, could effectively spreading surface without damaging osteogenic differentiation. Conclusion: We determined MP/TNT samples MP125) have excellent osteogenesis properties, may better application prospects.