<p>Nanodelivery and anticancer effect of a limonoid, nimbolide, in breast and pancreatic cancer cells</p>

• DOI: 10.2147/ijn.s208540
• Publication Date: 2019-10-06T19:01:39Z
• Authors: Arjun Patra; Swaha Satpathy; Muhammad Delwar H...
• Keywords: PLGA; Azadirachta; PEGylation

Nimbolide (Nim), a limonoid obtained from the neem tree, Azadirachta indica, has several pharmacological properties, including anticancer effects in different type of cancers. No drug-delivery system been reported for enhancing therapeutic application this novel hydrophobic molecule.In present research, poly(lactic-co-glycolic acid) (PLGA) nanoparticles Nim (Nim-nano) were formulated by nanoprecipitation, characterized physicochemical and screened potential breast (MCF-7 MDA-MB-231) pancreatic (AsPC-1) cancer cell lines.The Nim-nano had particle size 183.73±2.22 nm 221.20±11.03 before after lyophilization, respectively. Cryoprotectants (mannitol sucrose) significantly inhibited growth due to lyophilization. The ζ-potential was -22.40±4.40 mV. Drug loading encapsulation efficiency 5.25%±1.12% 55.67%±12.42%, exhibited sustained release more than 6 days PBS (pH 7.4) showed two- three-fold enhanced cytotoxicity lines compared with free Nim.The formulation great treatment cancers, such as cancer. Further, PLGA-polymer surface can be modified conjugation polyethylene glycol, receptor-binding ligands (eg, folic acid), other that which may lead targeted delivery