<p>Anti-MAdCAM-1-Conjugated Nanocarriers Delivering Quantum Dots Enable Specific Imaging of Inflammatory Bowel Disease</p>

• DOI: 10.2147/ijn.s264513
• Publication Date: 2020-11-01T20:02:11Z
• Authors: Marta Truffi; Marta Sevieri; Lucia Morelli; Matteo Monieri; Serena Mazzucchelli; Luca Sorrentino; Raffaele Allevi; Arianna Bonizzi; Pietro Zerbi; Beatrice Marchini; Erika Longhi; Gianluca Matteo S...; Francesco Colombo; Davide Prosperi; Miriam Colombo; Fabio Corsi
• Keywords: Addressin; Biodistribution; Ex vivo; Nanocarriers; Molecular Imaging

Assessment of inflammatory bowel disease (IBD) currently relies on aspecific clinical signs inflammation. Specific imaging the diseased regions is still lacking. Here, we investigate mucosal addressin cell adhesion molecule 1 (MAdCAM-1) as a reliable and specific endothelial target for engineered nanoparticles delivering agents to obtain an exact mapping foci.We generated nanodevice composed PLGA-PEG coupled with anti-MAdCAM-1 antibody half-chains loaded quantum dots (P@QD-MdC NPs). Bowel localization systemic biodistribution nanoconjugate were analyzed upon injection in murine model chronic IBD obtained through repeated administration dextran sulfate sodium salt. Specificity was also assessed ex vivo human specimens from patients IBD. Potential development contrast agent magnetic resonance by preliminary study animal model.Synthesized revealed good stability monodispersity. Molecular targeting properties vitro culture model. Upon intravenous injection, P@QD-MdC NPs localized colitic mice, enhanced accumulation at 24 h post-injection compared untargeted (p<0.05). Nanoparticles did not induce histologic lesions non-target organs. Ex exposure recognition vs uninvolved tracts (p<0.0001). After loading appropriate agent, enabled enhancement mucosa rectum treated mice.P@QD-MdC efficiently detected inflammation foci, accurately following expression pattern MAdCAM-1. Fine-tuning this offers promising non-invasive tool diagnosis.