<p>Creation of Bony Microenvironment with Extracellular Matrix Doped-Bioactive Ceramics to Enhance Osteoblast Behavior and Delivery of Aspartic Acid-Modified BMP-2 Peptides</p>

• DOI: 10.2147/ijn.s272571
• Publication Date: 2020-10-28T23:45:16Z
• Authors: Jinge Zhou; Zekang Xiong; Man Liu; Liang Yang; Sheng Yao; Kaifang Chen; Keda Yu; Yanzhen Qu; Tingfang Sun; Xiaodong Guo
• Keywords: Bioactive Glass

Decellularized matrix from porcine small intestinal submucosa (SIS) endows scaffolds with an ECM-like surface, which enhances stem cell self-renewal, proliferation, and differentiation. Mesoporous bioactive glass (MBG) is extensively recognized as excellent bio-ceramic for fabricating bone grafts.In the current study, SIS was doped on MBG scaffold (MBG/SIS) using polyurethane foam templating polydopamine chemistry method. To mimic bony environment of a natural matrix, ECM-inspired delivery system constructed by coupling BMP2-related peptide P28 to heparinized MBG/SIS (MBG/SIS-H-P28). The release MBG/SIS-H-P28 its effects viability, osteogenic differentiation marrow stromal cells were investigated in vitro vivo.Our research indicated that novel tissue-derived ECM has hierarchical interconnected porous architecture, superior biomechanical properties. released controlled manner, long-term time 40 d. results experiments showed improvements alkaline phosphatase activity, mRNA expression levels osteogenesis-related genes (Runx-2, OCN, OPN, ALP) compared those or MBG/SIS-P28 MBG/SIS-H-P28. vivo demonstrated evidently increased formation rat calvarial critical-sized defect controls.MBG/SIS-H-P28 show potential ideal platforms providing regeneration.