<p>Nanoscale Thermosensitive Hydrogel Scaffolds Promote the Chondrogenic Differentiation of Dental Pulp Stem and Progenitor Cells: A Minimally Invasive Approach for Cartilage Regeneration</p>

• DOI: 10.2147/ijn.s274418
• Publication Date: 2020-10-11T20:44:05Z
• Authors: Wael Talaat; Smriti Aryal AC; Sausan Al Kawas; AB Rani Samsudin; Nadia G Kandile; David RK Harding; Mohamed M Ghoneim; Waleed Zeiada; Jayalakshmi Jagal; Ahmed Aboelnaga; Mohamed Haider
• Keywords: Chondrogenesis; Nanocellulose; Biocompatibility

Several scaffolds and cell sources are being investigated for cartilage regeneration. The aim of the study was to prepare nanocellulose-based thermosensitive injectable hydrogel assess their potential as 3D allowing chondrogenic differentiation embedded human dental pulp stem progenitor cells (hDPSCs).The hydrogel-forming solutions were prepared by adding β-glycerophosphate (GP) chitosan (CS) at different ratios. Nanocellulose (NC) suspension produced from hemp hurd then added dropwise CS/GP mixture. In vitro characterization hydrogels involved optimizing gelation degradation time, mass-swelling ratio, rheological properties. with optimal characteristics, NC-CS/GP-21, selected further investigation including assessment biocompatibility. chondrogenesis ability hDPSCs in NC-CS/GP-21 compared that bone marrow-derived mesenchymal (BMSCs), confirmed vivo 12 adult Sprague Dawley rats.The showed stability culture media, had a time 2.8 minutes, highly porous microstructure scanning electron microscope, morphologically intact 14 days after injection. Histological immunohistochemical analyses real-time PCR hydrogel.Our results suggest nanocellulose-chitosan is biocompatible, injectable, mechanically stable slowly degradable scaffold. promising, minimally invasive, cell-based strategy