Interleukin-1β-Treated Mesenchymal Stem Cells Inhibit Inflammation in Hippocampal Astrocytes Through Exosome-Activated Nrf-2 Signaling

• DOI: 10.2147/ijn.s289914
• Publication Date: 2021-02-21T20:30:13Z
• Authors: Kai Liu; Guo-Liang Cai; Zhe Zhuang; Si-Ying Pei; Sheng-Nan Xu; Ya-Nan Wang; Hong Wang; Xin Wang; Cheng Cui; Man-Chao Sun; Si-Hui Guo; Kun-Ping Jia; Xiu-Zhen Wang; Guo-Feng Cai
• Keywords: Astrogliosis

Background: Interleukin-1β (IL-1)-treated mesenchymal stem cells (MSCs) and IL-1-MSCs-conditioned medium (CM) exert anti-inflammatory roles. Astrocytes are essential for the modulation of synaptic activity neuronal homeostasis in brain. Exosomes critical mediators intercellular communication. However, mechanism underlying effect IL-1-treated MSCs remains unknown. Methods: In this study, exosomes (IL-1-Exo) were isolated from MSCs. addition, lipopolysaccharide (LPS)-treated hippocampal astrocytes status epilepticus (SE) mice treated with IL-1-Exo. Inflammatory activity, astrogliosis, cognitive performance measured to determine IL-1-Exo on inflammation. Results: The results revealed that significantly inhibited LPS-induced astrogliosis inflammatory responses astrocytes. Also, reversed calcium signaling. Nrf2 signaling pathway was associated LPS-treated Furthermore, reduced response improved SE mice. Conclusion: suggest primarily mediated through Nrf-2 pathway. This study provides a new understanding molecular inflammation-associated brain diseases an avenue develop nanotherapeutic agents treatment conditions Keywords: cells, interleukin-1β, exosome, astrocyte, inflammation,