Self-Assembled DNA Nanostructure as a Carrier for Targeted siRNA Delivery in Glioma Cells

• DOI: 10.2147/ijn.s295598
• Publication Date: 2021-03-02T21:23:25Z
• Authors: Yanghao Zhou; Qiang Yang; Feng Wang; Zunjie Zhou; Jing Xu; Si Cheng; Yuan Cheng
• Keywords: 0301 basic medicine; Medicine (General); Cell Survival; Survivin; Down-Regulation; Apoptosis; rna interference; 03 medical and health sciences; R5-920; International Journal of Nanomedicine; Cell Line, Tumor; Human Umbilical Vein Endothelial Cells; Humans; Gene Silencing; RNA, Small Interfering; Original Research; Nucleolin; Cell Death; tumor targeting; apoptosis; Gene Transfer Techniques; dna tetrahedron; aptamer; RNA-Binding Proteins; DNA; Glioma; Aptamers, Nucleotide; Phosphoproteins; nanomedicine; Endocytosis; Nanostructures; 3. Good health; Oligodeoxyribonucleotides

RNA interference is a promising therapy in glioma treatment. However, the application of has been limited by instability and lack tumor targeting. Here, we report novel DNA tetrahedron, which can effectively deliver small interfering to cells induce apoptosis. siRNA, that suppress expression survivin glioma, was loaded into tetrahedron (TDN). To enhance ability active targeting this nanoparticle, modified one side nanostructure with aptamer as1411 (As-TDN-R), selectively recognize nucleolin cytomembrane cells. The nanoparticles were characterized agarose gel electrophoresis, dynamic light scattering, transmission electron microscopy. serum stability evaluated electrophoresis. Nucleolin detected Western blot immunofluorescence, targeted cellular uptake examined flow cytometry. TUNEL assay, cytometry, Blot used detect apoptosis U87 gene silencing qPCR, Blot, immunofluorescence. As-TDN-R alone showed better towards indicating TDN good siRNA protector. Compared alone, there increased intercellular cells, evidenced overexpressed cell lines. revealed group. downregulation protein mRNA levels indicated silenced target gene. nanoparticle serve as carrier for delivery glioma. Further exploration greatly promote DNA-based drug systems