The Effects of Luminescent CdSe Quantum Dot-Functionalized Antimicrobial Peptides Nanoparticles on Antibacterial Activity and Molecular Mechanism

• DOI: 10.2147/ijn.s295928
• Publication Date: 2021-03-04T22:06:43Z
• Authors: Wanzhen Li; Ping Song; Ying Xin; Zhao Kuang; Qin Liu; Fei Ge; Longbao Zhu; xuguang zhang; Yugui Tao; Weiwei Zhang
• Keywords: Pore Forming Cytotoxic Proteins; 0301 basic medicine; Medicine (General); Luminescence; Colony Count, Microbial; Mice, Nude; Apoptosis; Bacteremia; Microbial Sensitivity Tests; 02 engineering and technology; 03 medical and health sciences; R5-920; International Journal of Nanomedicine; Quantum Dots; Cadmium Compounds; Escherichia coli; Animals; Original Research; Gene Expression Profiling; antibacterial peptides; Gene Expression Regulation, Bacterial; Endocytosis; Anti-Bacterial Agents; 3. Good health; Disease Models, Animal; in vitro and in vivo; Gene Ontology; Microscopy, Fluorescence; antibacterial mechanism; cdse qd; rnaseq; Nanoparticles; Female; 0210 nano-technology

Background: With the development of bacterial resistance, range effective antibiotics is increasingly becoming more limited.The use nanoscale antimicrobial peptides (AP) in therapeutic and diagnostic methods a strategy for new antibiotics.Methods: Combining both AP cadmium selenide (CdSe) into composite material may result reagent with novel properties, such as enhanced antibacterial activity, fluorescence favorable stability aqueous solution.Results: AP-loaded CdSe NPs (AP-CdSe NPs) showed strong activity against multidrug-resistant (MDR) Escherichia coli (E.coli) Staphylococcus aureus (S. aureus) vitro vivo.Colony-forming unit (CFU) minimum inhibitory concentration (MIC) assays that AP-CdSe have highly activity.The quantitative analysis apoptosis by flow cytometry further confirmed MDR E. S. treated had death rates 98.76% 99.13%, respectively.Also, was found to inhibit an vivo bacteremia model mice infected aureus.In addition, mechanism determined RNA sequencing analysis.Gene ontology (GO) Kyoto encyclopedia genes genomes (KEGG) pathway revealed molecular effect NPs.Importantly, histopathology analysis, hematological toxicity indicated few side effects. Conclusion:These results demonstrate loaded on higher water solubility, bioavailability compared raw AP.This study reports findings are helpful design treatment strategies based AP.