Soil Fungi as Biomediator in Silver Nanoparticles Formation and Antimicrobial Efficacy

• DOI: 10.2147/ijn.s356724
• Publication Date: 2022-06-29T09:40:19Z
• Authors: Hana Sonbol; Afrah Mohammed; Shereen M Korany
• Keywords: Silver nanoparticle; Chaetomium globosum; Chaetomium

Biogenic agents in nanoparticles fabrication are gaining great interest due to their lower possible negative environmental impacts. The present study aimed isolate fungal strains from deserts Saudi Arabia and assess ability silver (AgNPs) evaluate antibacterial effect.Soil fungi were identified using 18s rDNA, NPs was assessed as extracellular synthesis, then UV-vis spectroscopy, dynamic light scattering (DLS), energy-dispersive X-ray transmission electron microscopy used for AgNPs characterization. activity of fungal-based against one Gram-positive methicillin-resistant S. aureus (MRSA) three Gram-negative bacteria (E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae). Ultrastructural changes caused by on K. pneumoniae investigated TEM along with SDS-PAGE protein profile patterns.The isolates Phoma sp. (MN995524), Chaetomium globosum (MN995493), (MN995550), filtrate reduced Ag ions into spherical P-AgNPs, G-AgNPs, C-AgNPs, respectively. DLS data showed an average size between 12.26 70.24 nm, where EDX spectrums represent at 3.0 keV peak. G-AgNPs displayed strong activities pneumoniae, the ultrastructural noted. Additionally, analysis treated revealed fewer bands compared control, which could be related degradation.Present findings have consequently developed eco-friendly approach formation environmentally isolated yield agents.