Smart Chondroitin Sulfate Micelles for Effective Targeted Delivery of Doxorubicin Against Breast Cancer Metastasis

0301 basic medicine Medicine (General) Mice, Inbred BALB C breast cancer therapy Skin Neoplasms Chondroitin Sulfates dual-targeting 02 engineering and technology 3. Good health Mice 03 medical and health sciences R5-920 Drug Delivery Systems Melanoma, Cutaneous Malignant International Journal of Nanomedicine Doxorubicin Cell Line, Tumor drug delivery metastasis Animals 0210 nano-technology reduction-sensitive micelles Micelles Original Research
DOI: 10.2147/ijn.s398802 Publication Date: 2023-02-09T18:05:09Z
ABSTRACT
Metastasis is a major challenge in breast cancer therapy. The successful chemotherapy of largely depends on the ability to block metastatic process. Herein, we designed dual-targeting and stimuli-responsive drug delivery system for targeted against metastasis.AS1411 aptamer-modified chondroitin sulfate A-ss-deoxycholic acid (ACSSD) was synthesized, unmodified CSSD used as control. Chemotherapeutic doxorubicin (DOX)-containing ACSSD (D-ACSSD) micelles were prepared by dialysis method. conjugate confirmed Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), dynamic light scattering (DLS), transmission electron microscopy (TEM). In vitro cellular uptake cytotoxicity D-ACSSD studied confocal laser scanning (CLSM) MTT assay tumor cells. inhibition capability cell migration invasion carried out 4T1 vivo antitumor activity DOX-containing investigated 4T1-bearing Balb/c mice.D-ACSSD DOX-loaded (D-CSSD) exhibited high encapsulation content reduction-responsive characteristics. spherical shape. Compared with D-CSSD, showed higher more potent killing MDA-MB-231 Additionally, stronger inhibitory effects highly cells than D-CSSD. Among formulations, presented most effective growth lung metastasis orthotopic mice vivo. It also revealed that did not exhibit obvious systemic toxicity.The smart could be promising therapy cancer.
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