Smart Chondroitin Sulfate Micelles for Effective Targeted Delivery of Doxorubicin Against Breast Cancer Metastasis

• DOI: 10.2147/ijn.s398802
• Publication Date: 2023-02-09T18:05:09Z
• Authors: Jingmou Yu; Xin Xie; Liangliang Wang; Wenbo Liu; Huifeng Xu; Xiangmei Lu; Xiaofan Li; Jin Ren; Weidong Li
• Keywords: 0301 basic medicine; Medicine (General); Mice, Inbred BALB C; breast cancer therapy; Skin Neoplasms; Chondroitin Sulfates; dual-targeting; 02 engineering and technology; 3. Good health; Mice; 03 medical and health sciences; R5-920; Drug Delivery Systems; Melanoma, Cutaneous Malignant; International Journal of Nanomedicine; Doxorubicin; Cell Line, Tumor; drug delivery; metastasis; Animals; 0210 nano-technology; reduction-sensitive micelles; Micelles; Original Research

Metastasis is a major challenge in breast cancer therapy. The successful chemotherapy of largely depends on the ability to block metastatic process. Herein, we designed dual-targeting and stimuli-responsive drug delivery system for targeted against metastasis.AS1411 aptamer-modified chondroitin sulfate A-ss-deoxycholic acid (ACSSD) was synthesized, unmodified CSSD used as control. Chemotherapeutic doxorubicin (DOX)-containing ACSSD (D-ACSSD) micelles were prepared by dialysis method. conjugate confirmed Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), dynamic light scattering (DLS), transmission electron microscopy (TEM). In vitro cellular uptake cytotoxicity D-ACSSD studied confocal laser scanning (CLSM) MTT assay tumor cells. inhibition capability cell migration invasion carried out 4T1 vivo antitumor activity DOX-containing investigated 4T1-bearing Balb/c mice.D-ACSSD DOX-loaded (D-CSSD) exhibited high encapsulation content reduction-responsive characteristics. spherical shape. Compared with D-CSSD, showed higher more potent killing MDA-MB-231 Additionally, stronger inhibitory effects highly cells than D-CSSD. Among formulations, presented most effective growth lung metastasis orthotopic mice vivo. It also revealed that did not exhibit obvious systemic toxicity.The smart could be promising therapy cancer.