Simple and Ultrasensitive Detection of Glioma-Related ctDNAs in Mice Serum by SERS-Based Catalytic Hairpin Assembly Signal Amplification Coupled with Magnetic Aggregation

• DOI: 10.2147/ijn.s410080
• Publication Date: 2023-06-14T04:10:08Z
• Authors: Youwei Wang; Yang Yang; Xiaowei Cao; Zhensheng Liu; Bing Chen; Qiu Du; Xiaoxia Lu
• Keywords: SIGNAL (programming language)

Purpose: Circulating tumor DNA (ctDNA) is more representative and accurate than biopsy also conducive to dynamic monitoring, facilitating diagnosis prognosis of glioma. Therefore, the present study aimed establish validate a novel amplified method for detection IDH1 R132H BRAF V600E, which were associated with genetic Patients Methods: A dual-signal amplification based on magnetic aggregation catalytic hairpin assembly (CHA) was constructed simultaneous ctDNAs. When target ctDNAs are present, CHA reaction initiated leads Au-Ag nanoshuttles (Au-Ag NSs) onto beads (MBs). Further enrichment MBs under an external field facilitated SERS. Results: The limit (LOD) V600E in serum as low 6.01 aM 5.48 aM. reproducibility selectivity proposed SERS analysis platform satisfactory. Finally, applied quantify subcutaneous-tumor‑bearing nude mice, results obtained by consistent those from quantitative real-time polymerase chain (qRT-PCR). Conclusion: showed that simple ultrasensitive strategy gliomas-associated detection, crucial early monitoring. Keywords: circulating DNA, nanoshuttles, beads, assembly, surface-enhanced Raman spectroscopy