Lactobacillus-Polydopamine System for Targeted Drug Delivery in Overactive Bladder: Evidence from Bladder Cell Spheroids, Rat Models, and Urinary Microbiome Profiling

• DOI: 10.2147/ijn.s465745
• Publication Date: 2024-08-15T04:50:15Z
• Authors: Xiaolong Wang; Guanyi Wang; Peibin Cen; Hongyu Lan; Linfa Guo; Zuhaer Yisha; Aodun Gu; Guiyong Liu; Zijian Wang; Tongzu Liu; Qingfeng Yu
• Keywords: Profiling (computer programming); Spheroid

Introduction:Overactive bladder (OAB) is a highly prevalent condition with limited treatment options due to poor efficacy, side effects, and patient compliance.Novel drug delivery systems that can target the wall may improve OAB therapy.Methods: We explored polydopamine (PDA)-coated lactobacillus platform as potential carrier for localized treatment.Urinary microbiome profiling was performed identify presence of in healthy groups.Lactobacillus-PDA nanoparticles were synthesized characterized by electron microscopy spectrophotometry.A rat perfusion model human smooth muscle cell spheroids used assess distribution penetration nanoparticles.The efficacy Lactobacillus-PDA system (LPS) delivering antimuscarinic solifenacin evaluated an model.Results: Urinary revealed dominant genus both groups.The exhibited uniform size optical properties.In model, distributed throughout without toxicity.The also penetrated spheroids.In LPS facilitated improved efficacy.Discussion: The results highlight promising targeted therapy via into tissues.This bacteriotherapy approach overcome limitations current systemic medications.Lactobacillus, probiotic bacterium present urinary tract microbiome, hypothesized adhere penetrate when coated PDA nanoparticles, making it suitable candidate delivery.