Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice

• DOI: 10.2147/ijn.s46997
• Publication Date: 2013-08-04T23:09:50Z
• Authors: Hsiao-Chi Chuang; Chien-Ling Su; Tzu-Tao Chen; Chih-Cheng Chang; Kai-Jen Chuang; Cheng-Kuan Wu; Wen-Te Liu; Kin Fai Ho; Kang-Yun Lee; Shu-Chuan Ho; Hsiu-Er Tseng; Tsun-Jen Cheng
• Keywords: Silver nanoparticle; Inhalation exposure

Background: Silver nanoparticles (AgNPs) have been associated with the exacerbation of asthma; however, immunological basis for adjuvant effects AgNPs is not well understood. Objective: The aim study reported here was to investigate allergic AgNP inhalation using proteomic approaches. Methods: Allergen provoked mice were exposed 33 nm at 3.3 mg/m 3 . Following this, bronchoalveolar lavage fluid (BALF) and plasma collected determine protein profiles. Results: In total, 106 79 AgNP-unique proteins identified in BALF control mice, respectively. Additionally, 40 26 found profiles suggested that metabolic, cellular, immune system processes pulmonary exposure AgNPs. addition, we observed 18 systemic lupus erythematosus commonly expressed both after exposure. Significant allergy responses as determined by ovalbumin-specific immunoglobulin E. Conclusion: Inhaled may regulate lungs mice. Our results suggest immunology a vital response Keywords: lavage, immunotoxicology, proteome, erythematosus, serum