Fabrication of Nano-Silver Composite Using Amomum longiligulare Fruit Polysaccharides and Their Biological Activities

• DOI: 10.2147/ijn.s501828
• Publication Date: 2025-02-11T14:30:12Z
• Authors: Yong Lian; Ning Ma; Qianying Cheng; Mingquan Luo; Zhen Xu; Fei He; Xiaomei Zhou; Ying Zhang; Dejun Jin; Yidan Kong; Yong Wang; Na Wei
• Keywords: Silver nanoparticle; ABTS; Silver nitrate; Minimum bactericidal concentration; Silver Nano

Purpose: Study aims to optimize the synthesis conditions for silver nanoparticle composites [ALP(D)-AgNPs] using a rapid and environmentally friendly method investigate antioxidant, antibacterial, anticancer activities of fabricated composite. Methods: The polysaccharide component ALP-D was extracted purified from fruits Amomum longiligulare subsequently used further experiments. structure characterized by FT-IR, monosaccharide composition molecular weight.The optimal green nanoparticles were determined through single-factor synthesized UV-Vis spectroscopy, DLS, HRTEM XRD. Finally, in vitro antioxidant activity, antibacterial activity nano-silver composite evaluated. Results: Single-factor experiments identified ALP(D)-AgNPs as reaction time 180 min, temperature 100 °C, 10:1 volume ratio nitrate ALP-D. free radical scavenging against DPPH ABTS significantly enhanced compared with that minimum inhibitory concentration (MIC) values E coli B subtilis 31.25 μg/mL, while MIC value S aureus 62.5 μg/mL. bactericidal (MBC) 125 μg/mL coli, subtilis, aureus. IC50 on MDA-MB-231, HepG2, Caco-2, C6 cancer cell lines 14.72 ± 0.23, 8.19 0.65, 22.73 3.01, 15.77 2.91 respectively. Conclusion: In summary, we have novel material nanoparticles. results show new exhibit excellent stability dispersibility. Furthermore, biological reveals possess notable cancer-suppressing activities. Keywords: ALP-D, ALP(D)-AgNPs,