Inhibition of A/Human/Hubei/3/2005 (H3N2) influenza virus infection by silver nanoparticles in vitro and in vivo

• DOI: 10.2147/ijn.s53622
• Publication Date: 2013-10-29T22:12:47Z
• Authors: Dongxi Xiang; Cong-long Zheng; Yang Zheng; Xiujing Li; Jianjian Yin; Michael O’ Conner; Manju Marappan; Yingqiu Miao; Bin Xiang; Wei Duan; Sarah Shigdar; Xiaojuan Zhao
• Keywords: Hemagglutination assay; Cytopathic effect

Abstract: Silver nanoparticles (AgNPs) have attracted much attention as antimicrobial agents and demonstrated efficient inhibitory activity against various viruses, including human immunodeficiency virus, hepatitis B Tacaribe virus. In this study, we investigated if AgNPs could antiviral preventive effects in A/Human/Hubei/3/2005 (H3N2) influenza virus infection. Madin-Darby canine kidney cells infected with AgNP-treated H3N2 showed better viability ( P <0.05 versus control) no obvious cytopathic compared an control group a treated the solvent used for preparation of AgNPs. Hemagglutination assay indicated that significantly inhibit growth <0.01 control). reduced cell apoptosis induced by at three different treatment pathways viruses were analyzed transmission electron microscopy found to interact each other, resulting destruction morphologic viral structures time-dependent manner time range 30 minutes 2 hours. addition, intranasal AgNP administration mice enhanced survival after infection Mice lower lung titer levels minor pathologic lesions tissue, had marked benefit during secondary passage vivo. These results provide evidence beneficial preventing both vitro vivo, demonstrate can be potential therapeutics inhibiting outbreaks influenza. Keywords: silver nanoparticles, H3N2, Corrigendum paper has been published.