Magnetic resonance hypointensive signal primarily originates from extracellular iron particles in the long-term tracking of mesenchymal stem cells transplanted in the infarcted myocardium

• DOI: 10.2147/ijn.s77858
• Publication Date: 2015-03-02T17:46:18Z
• Authors: Junbo Ge; Zheyong Huang; Chenguang Li; Shan Yang; Jianfeng Xu; Yunli Shen; Xinxing Xie; Yuxiang Dai; Hao Lu; Hui Gong; Aijun Sun; Juying Qian
• Keywords: Cardiac magnetic resonance

The long-lasting hypointensities in cardiac magnetic resonance (CMR) were believed to originate from superparamagnetic iron oxide (SPIO)-engulfed macrophages during long-term stem cell tracking. However, the clearance capacity of ischemic heart was limited. Therefore, we speculated that extracellular SPIO particles may also be involved generation false-positive signals.Male swine mesenchymal cells (MSCs) incubated with for 24 hours, and labeling had no significant effects on either viability or differentiation. In vitro studies showed failed distinguish living SPIO-MSCs dead SPIO-MSCs. Two hours after establishment female acute myocardial infarction model, 2×10(7) male SPIO-labeled MSCs (n=5) unlabeled transextracardially injected into infarcted myocardium at ten distinct sites. vivo CMR T2 star weighted imaging-flash-2D sequence revealed a signal void corresponding initial SPIO-MSC injection At 6 months transplantation, identified 32 (64%) 50 sites, where massive Prussian blue-positive deposits detected by pathological examination. predominantly distributed space, minority within CD68-positive other CD68-negative cells. No sex-determining region Y DNA donor detected.CMR hypointensive is primarily caused tracking transplanted infarction. Consideration should given both potential toxicity long-standing heart.