Osteoinductive peptide-functionalized nanofibers with highly ordered structure as biomimetic scaffolds for bone tissue engineering

• DOI: 10.2147/ijn.s94045
• Publication Date: 2015-11-18T23:17:34Z
• Authors: Shicheng Wei; Xiang Gao; Xiaohong Zhang; Jinlin Song; Xiao Xu; Anxiu Xu; Mengke Wang; Bingwu Xie; Enyi Huang; Feng Deng
• Keywords: Biomimetic Materials

Abstract: The construction of functional biomimetic scaffolds that recapitulate the topographical and biochemical features bone tissue extracellular matrix is now topical interest in engineering. In this study, a novel surface-functionalized electrospun polycaprolactone (PCL) nanofiber scaffold with highly ordered structure was developed to simulate critical native via single step catechol chemistry. Specially, under slightly alkaline aqueous solution, polydopamine (pDA) coated on surface aligned PCL nanofibers after electrospinning, followed by covalent immobilization morphogenetic protein-7-derived peptides onto pDA-coated surface. Contact angle measurement, Raman spectroscopy, X-ray photoelectron spectroscopy confirmed presence pDA Our results demonstrated modification osteoinductive could improve cytocompatibility terms cell adhesion, spreading, proliferation. Most importantly, Alizarin Red S staining, quantitative real-time polymerase chain reaction, immunostaining, Western blot revealed human mesenchymal stem cells cultured exhibited enhanced osteogenic differentiation potential than randomly oriented nanofibers. Furthermore, direct even absence osteoinducting factors, suggesting superior efficacy design combines advantages peptide signal fate decision. presented peptide-decorated bone-mimic hold promising context Keywords: biomimetic, nanofiber, peptide, engineering