Endothelin-1/Endothelin Receptor Type A-Angiopoietins/Tie-2 Pathway in Regulating the Cross Talk Between Glomerular Endothelial Cells and Podocytes in Trichloroethylene-Induced Renal Immune Injury

• DOI: 10.2147/jir.s301104
• Publication Date: 2021-03-08T20:57:58Z
• Authors: Haibo Xie; Hui Wang; Qifeng Wu; Jiale Peng; Hua Huang; Yican Wang; Meng Huang; Wei Jiang; Yi Yang; Xuesong Zhang; Jiaxiang Zhang; Qixing Zhu
• Keywords: Nephrin

This study aimed to investigate the mechanism in regulating cross talk between glomerular endothelial cells and podocytes "occupational medicamentosa-like dermatitis induced by trichloroethylene (OMLDT)" patients.Totally 6 OMLDT patients, 18 controls, 102 BALB/c female mice were involved this study. Patient's serum endothelin-1 (ET-1), angiopoietin-1 (Ang-1) angiopoietin-2 (Ang-2), blood urea nitrogen (BUN), podocalyxin (PCX) detected. All used establish (TCE) sensitized mouse model. Transmission electron microscope results reflect renal glomerulus injury. Protein levels detected Western blot. Ang-1/Ang-2 gene level was reflected RT-PCR. Cell apoptosis using TUNEL assay kit.We found that ET-1, Ang-2, BUN, PCX highly expressed but Ang-1 inhibited. In TCE positive mouse, downregulation of Ang-1, pTie-2 upregulation Ang-2 mediated ET-1/ETAR not ET-1/ETBR. The promotor proteins downregulated inhibitor upregulated treating with BQ123.ET-1/ETAR-Angs/Tie-2 pathway podocytes. BQ123 can alleviate immune