Jiawei Qifuyin Enhances Immunity and Improves Cognitive Impairment in APP/PS1 Mice Through Modulation of Neuroinflammatory Pathways

• DOI: 10.2147/jir.s479899
• Publication Date: 2024-11-18T07:25:13Z
• Authors: Jiahua Cheng; Wujuan Li; Lanlin Wang; Yu Gao; Yueran Ma; Min Zhou; Tian Yang; Changwu Yue; Long Yan; Yuhong Lyu
• Keywords: intestinal microbiota; 0301 basic medicine; 0303 health sciences; short chain fatty acid; RM1-950; jiawei qifuyin; neuroinflammation; Pathology; alzheimer's disease; RB1-214; Therapeutics. Pharmacology; Original Research

PURPOSE: To explore the protective effects and mechanisms of Jiawei Qifuyin on APP/PS1 mice, a model for Alzheimer’s disease (AD). METHODS: Network pharmacology tools were used to predict anti-AD targets and signaling pathways affected by Jiawei Qifuyin. In vitro studies assessed antioxidant and oxygen radical scavenging abilities, immune cell proliferation, and inflammatory cytokine levels in lipopolysaccharide-induced BV2 microglial cells. Cognitive ability in APP/PS1 mice was evaluated using the Morris Water Maze test. mRNA expression of neuroinflammatory factors, changes in intestinal microbiota, and short-chain fatty acid content were analyzed post-treatment. RESULTS: Network pharmacology predicted that Jiawei Qifuyin affects AKT1, TNF-α, and AGE/RAGE pathways. It showed concentration-dependent antioxidant effects and modulated immune cell proliferation. IL-2, IL-6, and TNF-α levels in LPS-induced BV2 cells were significantly reduced. Treated animals exhibited improved cognitive performance, decreased brain amyloid-beta levels, and downregulated expression of IL-1β, IL-6, RAGE, and NF-κB. Significant changes in intestinal microbiota composition and SCFA content were observed. CONCLUSION: Jiawei Qifuyin may enhance immunity and improve cognitive impairment in APP/PS1 mice through regulation of inflammatory factors, gut microbiota, and the gut-brain axis.