Compound 21 Attenuates Isoflurane-Induced Injury in Neonatal Rat Hippocampal Neurons and Primary Rat Neuronal Cells by Upregulating METTL3

• DOI: 10.2147/jir.s483211
• Publication Date: 2024-11-30T06:00:11Z
• Authors: Yaping Shen; Yijiu Wu; Hai Zhuang; Zhumei Chen; Qiong Zhang; Peixin Li; Jing Wang; Zhi Huang; Qingfan Zeng
• Keywords: Primary (astronomy)

Isoflurane, as an anesthetic drug, has a neurotoxic effect on the developing brain tissue. Compound 21 (C21) been reported to be neuroprotective and ameliorate stroke effects. However, mechanism by which C21 protects against nerve damage remains unclear. Animal cellular models of injury were constructed using isoflurane (ISO) in neonatal SD rats primary rat neuronal cells (PRNCs). After treatment with C21, ultrastructure morphology hippocampus model assessed transmission electron microscope H&E staining. Methylation or apoptosis-related genes proteins examined immunohistochemistry, RT-qPCR, Western blot. The levels inflammatory factors monitored ELISA kits. m6A modification was analyzed Dot blot MeRIP-qPCR. Cell proliferation apoptosis also tested Edu TUNEL suppresses inflammation improves hippocampal ISO-induced rats. Mechanistically, upregulates modification, PPAR-a, BCL-2, METTL3 ISO-treated PRNCs. promotes cell proliferation, enhances BCL-2 reduces upregulating These findings suggest that survival up-regulates methylation Bc1-2 levels, potentially offering therapeutic strategy for neuroprotection clinical settings, particularly cases exposure. may related upregulation METTL3.