Hypoxic and ischemic effects on gene and protein expression levels of paracrine factors by human olfactory mucosa mesenchymal-like stem cells

• DOI: 10.2147/jn.s118538
• Publication Date: 2016-12-08T02:30:21Z
• Authors: Ting Yuan; Yi Zhuo; Chaying Su; Xuan Li; Da Duan; Lite Ge; Pei Wu; Hao Wang; Yujia Deng; Ming Lu
• Keywords: Olfactory mucosa

Abstract: Human olfactory mucosa mesenchymal-like stem cells (hOM-MSCs) secrete paracrine factors that may exert a protective effect on the cerebral ischemia. This study was done to determine hypoxic and ischemic effects mRNA protein expression level of by hOM-MSCs. The hOM-MSCs were cultured with 5% or 20% serum under either normoxic (21% O 2 ) (3% conditions. Expression levels for vascular endothelial growth factor (VEGF), nerve (NGF), brain-derived neurotrophic (BDNF), glial cell line-derived (GDNF) matrix metalloproteinase-2 (MMP-2) determined quantitative reverse transcription polymerase chain reaction Western blot, respectively. Hypoxia reduced gene VEGF (5% serum), GDNF, BDNF serum) NGF increased it (20% MMP-2 serum). Ischemia lowered (hypoxia), (normoxia) (hypoxia) (normoxia), (normoxia).The these almost in line level. These data demonstrate oxygen have significant hOM-MSCs, which will affect how interact vivo during Keywords: human cells, factors, hypoxia, ischemia, ischemia