Low-Dose Interleukin-2 and Regulatory T Cell Treatments Attenuate Punctate and Dynamic Mechanical Allodynia in a Mouse Model of Sciatic Nerve Injury

• DOI: 10.2147/jpr.s301343
• Publication Date: 2021-04-05T23:45:33Z
• Authors: Rong Hu; Jintao Zhang; Xuemei Liu; Dong Huang; Yu-Qing Cao
• Keywords: SNi; Allodynia; Adoptive Cell Transfer; Nerve Injury; Regulatory T cell

Nerve injury-induced mechanical hyper-sensitivity, in particular stroking-induced dynamic allodynia, is highly debilitating and difficult to treat. Previous studies indicate that the immunosuppressive regulatory T (Treg) cells modulate magnitude of punctate allodynia resulting from sciatic nerve injury. However, whether enhancing Treg-mediated suppression attenuates not known. In present study, we addressed this knowledge gap by treating mice with low-dose interleukin-2 (ld-IL2) injections or adoptive transfer Treg cells. Female Swiss Webster received daily ld-IL2 (1 μg/mouse, intraperitoneally) either before after unilateral spared injury (SNI). Male C57BL/6J 1 x 106 3 weeks post-SNI. The responses stimuli on hindpaw were monitored up 4-6 We also compared distribution CD3+ total SNI and/or treatment. Ld-IL2 pretreatment female completely blocked development SNI-induced reduced allodynia. Delayed treatment significantly attenuated morphine-resistant at 3-5 Adoptive male post-SNI effectively reversed persistent supporting effect mediated through endogenous cells, likely independent mouse strain sex. Neither nor affected basal brush stimuli. increased frequency among injured nerves but uninjured dorsal root ganglia, suggesting as ld-IL2's site action. Collectively, results study supports a cellular target potential therapeutic option for